Transfer Buffer ( for Western blotting )

Transfer Buffer (for Western blotting) - 2 L

25 mM Tris, 192 mM glycine, 10% methanol

	1X Transfer Buffer	10X Transfer Buffer
	Reagents needed:	Reagents needed:

28.8 g	glycine	288 g	glycine
6.04 g	Tris base	60.4 g	Tris base
200 ml	methanol	-	methanol
1.6 L	ddH₂O	1.8 L	ddH₂O

** NOTE: for the proper transfer of large proteins, up to 0.5% SDS may need to be added to 1X Transfer Buffer. **

Directions for 1X Transfer Buffer:

1) Dissolve Tris base and glycine together in 1.6 L of ddH₂O.

2) Add methanol and mix.

3) Add ddH₂O to a final volume of 2 L.

Directions for 10X Transfer Buffer:

1) Dissolve Tris base and glycine together in 1.8 L of ddH₂O.

2) Add ddH₂O to a final volume of 2 L.

** To make 1X Transfer Buffer from 10X: Mix 100 ml of 10X Transfer Buffer, 100 ml of methanol and 800 ml of ddH₂O per liter **

© 2007 Joseph T.E. Roland
www.Cytographica.com