Cold Water Fish Skin Gelatin Blocking Buffer

0.5% Fish Skin Gelatin Blocking Buffer - ml

0.5% of Cold Water Fish Skin Gelatin (Teleostean Gelatin)
in 1X phosphate buffered saline - pH 7.4 with 0.1% Triton X-100 and 0.1% casein (optional)

Reagents needed:

1	ml Teleostein Gelatin ∼45% in ddH₂O (Sigma Cat# G7765)
0.5	ml 20% Triton X-100
10	ml 10X PBS
0.1	g Casein (Enzymatic Hydrolysate) OPTIONAL
88.5	ml ddH₂O

Directions:

1) Add 10 ml of 10X PBS to 88.5 ml of ddH₂O.

2) Add 1 ml of Cold Water Fish Skin Gelatin (Teleostein Gelatin) ∼45% in ddH₂O.

3) (OPTIONAL) Add 0.1 g of Casein

4) Add 0.5 ml of 20% Triton X-100 and mix.

5) Filter through a 0.45 μm filter.

6) Aliquot and freeze at -20 °C for long-term storage.

© 2008 Joseph T.E. Roland
www.Cytographica.com