YPDA (yeast peptone dextrose adenine) Media - 500 ml

Reagents needed:

10 g
Bacto yeast extract
20 g
Bacto peptone
20 g
Glucose monohydrate
40 mg
Adenine hemisulfate
20 g
Bacto Agar (only for making YPDA/agar plates)
800 ml
ddH2O

Directions:

1) Dissolve all ingredients into 800 ml of ddH2O by stirring.

** NOTE: if making YPDA/agar plates, add 20 g of Bacto Agar now **

2) Add ddH2O until final volume is 1 L.

3) Autoclave to sterilize.

6) Store at 4°C.

 

© 2007 Joseph T.E. Roland
www.Cytographica.com