TE (Tris-EDTA) buffer (pH 7.4 to 8.0) - 1L

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10 mM Tris, 1 mM EDTA - pH is determined by the pH of the Tris-Cl

1X TE	10X TE
Reagents needed:	Reagents needed:

10 ml	1 M Tris-Cl	100 ml	1 M Tris-Cl
2 ml	0.5 M EDTA pH 8.0	20 ml	0.5 M EDTA pH 8.0
988 ml	ddH2O	880 ml	ddH2O

Directions:

1) Mix Tris-Cl and EDTA with ddH₂O.

2) Sterilize by autoclaving.

3) Store at 4°C or room temperature.

© 2007 Joseph T.E. Roland
www.Cytographica.com