TBST (Tris-buffered saline with Tween) - 500 ml

0.1% Tween-20 in 1X Tris-buffered saline - pH 7.4

Reagents needed:

2.5 ml
20% Tween-20
50 ml
10X TBS
447.5 ml
ddH2O

Directions:

1) Add 50 ml of 10X TBS to the 447.5 ml of ddH2O.

2) Add 2.5 ml of 20% Tween-20 and mix.

 

 

© 2007 Joseph T.E. Roland
www.Cytographica.com