TBS (Tris-buffered saline)

TBS (Tris-buffered saline) - 1L

25 mM Tris, 150 mM NaCl, 2 mM KCl, pH 7.4

	1X TBS	10X TBS
	Reagents needed:	Reagents needed:

8 g	NaCl	80 g	NaCl
0.2 g	KCl	2 g	KCl
3 g	Tris base	30 g	Tris base
800 ml	ddH₂O	800 ml	ddH₂O

Directions:

1) Dissolve all dry reagents together in 800 ml of ddH₂O.

2) Adjust the pH to 7.4 using HCl.

3) Add ddH₂O to a final volume of 1 L.

4) Sterilize by autoclaving.

© 2007 Joseph T.E. Roland
www.Cytographica.com