TBE (Tris-borate-EDTA) - 1L

45 mM Tris-borate, 1 mM EDTA

  1X TBE 5X TBE
  Reagents needed: Reagents needed:
     
4.84 g
Tris base
54 g
Tris base
5.5 g
boric acid
27.5 g
boric acid
2 ml
0.5 M EDTA pH 8.0
10 ml
0.5 M EDTA pH 8.0
 
900 ml
ddH2O
900 ml
ddH2O

Directions:

1) Dissolve Tris base in 800 ml of ddH2O by stirring.

2) Add boric acid and mix.

3) Add EDTA and mix.

4) Add ddH2O to a final volume of 1 L.

5) Sterilize by autoclaving.

 

© 2007 Joseph T.E. Roland
www.Cytographica.com