TBE (Tris-borate-EDTA)

TBE (Tris-borate-EDTA) - 1L

45 mM Tris-borate, 1 mM EDTA

	1X TBE	5X TBE
	Reagents needed:	Reagents needed:

4.84 g	Tris base	54 g	Tris base
5.5 g	boric acid	27.5 g	boric acid
2 ml	0.5 M EDTA pH 8.0	10 ml	0.5 M EDTA pH 8.0
900 ml	ddH₂O	900 ml	ddH₂O

Directions:

1) Dissolve Tris base in 800 ml of ddH₂O by stirring.

2) Add boric acid and mix.

3) Add EDTA and mix.

4) Add ddH₂O to a final volume of 1 L.

5) Sterilize by autoclaving.

© 2007 Joseph T.E. Roland
www.Cytographica.com