TAE (Tris-acetate-EDTA)

TAE (Tris-acetate-EDTA) - 1L

40 mM Tris-acetate, 1 mM EDTA

	1X TAE	50X TAE
	Reagents needed:	Reagents needed:

4.84 g	Tris base	242 g	Tris base
1.14 ml	glacial acetic acid	57.1 ml	glacial acetic acid
2 ml	0.5 M EDTA pH 8.0	100 ml	0.5 M EDTA pH 8.0
800 ml	ddH₂O	800 ml	ddH₂O

Directions:

1) Dissolve Tris base in 800 ml of ddH₂O by stirring.

2) Add glacial acetic acid and EDTA and mix.

3) Add ddH₂O to a final volume of 1 L.

4) Sterilize by autoclaving.

© 2007 Joseph T.E. Roland
www.Cytographica.com