LBE-5052 Autoinduction Media - 1000 ml


1% Bacto-tryptone, 0.5% Bacto-yeast extract, 40 mM K2HPO4, 10 mM KH2PO4, 50 mM NH4Cl, 5 mM Na2SO4, 2 mM Mg2SO4, 0.5% glycerol, 0.05% glucose, 0.2% lactose, trace metals

1) Mix together in a 2 Liter Erlenmeyer flask :

898 ml ddH2O
10 g Bacto-tryptone
5 g Bacto-yeast extract
2.68 g NH4Cl
0.71 g Na2SO4
g Glycerol
0.5 g Glucose
2 g Lactose

2) Autoclave using a Liquid cycle for 45 minutes.

3) Cool to room temperature, then add:

1 ml 2 M MgSO4  (0.22Ám FILTER STERILIZED)
1 ml 1000X Trace Metals Mix  (0.22Ám FILTER STERILIZED)
40 ml 1 M K2HPO4  (0.22Ám FILTER STERILIZED or AUTOCLAVED)
10 ml 1 M KH2PO4  (0.22Ám FILTER STERILIZED or AUTOCLAVED)

4) Add the appropriate antibiotic(s) and the media is ready to use.

Based on "Protein production by auto-induction in high-density shaking cultures", F. William Studier,
Protein Expression and Purfication, 41 (2005) 207-234

© 2011 Joseph T.E. Roland
www.Cytographica.com