Kanamycin - 10 mL (1,000 X Stock)
50 mg/ml kanamycin in water
Reagents needed:
0.5 g |
kanamycin disulfate salt |
10 ml |
ddH2O |
Directions:
1) Dissolve 0.5 g of kanamycin into 10 ml of ddH2O.
2) Filter through a 0.22 µm filter to sterilize.
3) Aliquot and store at -20°C.
4) Use at 1:1000 dilution in LB or LB-Agar.
© 2007 Joseph T.E. Roland
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