BLOTTO (Bovine Lacto Transfer Technique Optimizer) - ml  

5% Non-fat dry milk and 0.1% Tween-20 in 1X Tris-buffered saline - pH 7.4

Reagents needed:

5
g Powdered Non-fat Dry Milk
0.5
ml 20% Tween-20
10
ml 10X TBS
ml ddH2O

Directions:

1) Add 10 ml of 10X TBS into 80 ml of ddH2O and mix.

2) Add 0.5 ml of 20% Tween-20 and mix.

3) Dissolve 5 g of powdered non-fat dry milk and mix.

4) Add ddH2O until final volume is 100 ml.

© 2007 Joseph T.E. Roland
www.Cytographica.com