Laemmli Sample Buffer - 20 ml


60 mM Tris-Cl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.01% bromophenol blue

5X Sample Buffer

Reagents needed:

4 ml
10 ml
glycerol
5 ml
β-mercaptoethanol
2 g
SDS (sodium dodecyl sulfate / sodium lauryl sulfate)
1 ml
1% bromophenol blue

** CAUTION ** β-mercaptoethanol and SDS powder are hazerdous. Prepare solution in a ventilated fume hood.

Directions:

1) Add 1 ml of 1% bromophenol blue to 4 ml of 1.5 M Tris-Cl pH 6.8.

2) Add 10ml of glycerol and mix.

3) Add 2 g of SDS and mix (the SDS will take a few minutes to dissolve).

4) Add 5 ml of β-mercaptoethanol and mix.

5) Aliquot and store at -20°C.

Alternatively, 250 µl of β-mercaptoethanol can be added just prior to use. Simply omit step 4 and the final volume will be 16 ml.

 

© 2007 Joseph T.E. Roland
www.Cytographica.com