Laemmli Sample Buffer - 20 ml
60 mM Tris-Cl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.01% bromophenol blue
5X Sample Buffer
Reagents needed:
4 ml |
|
10 ml |
glycerol |
5 ml |
β-mercaptoethanol |
2 g |
SDS (sodium dodecyl sulfate / sodium lauryl sulfate) |
1 ml |
1% bromophenol blue |
** CAUTION ** β-mercaptoethanol and SDS powder are hazerdous. Prepare solution in a ventilated fume hood.
Directions:
1) Add 1 ml of 1% bromophenol blue to 4 ml of 1.5 M Tris-Cl pH 6.8.
2) Add 10ml of glycerol and mix.
3) Add 2 g of SDS and mix (the SDS will take a few minutes to dissolve).
4) Add 5 ml of β-mercaptoethanol and mix.
5) Aliquot and store at -20°C.
Alternatively, 250 µl of β-mercaptoethanol can be added just prior to use. Simply omit step 4 and the final volume will be 16 ml.
© 2007 Joseph T.E. Roland
www.Cytographica.com