Calculate Polyacrylamide gel recipes for SDS-PAGE

Just enter the number of gels (18x16mm) and the percent polyacrylamide needed

 
Enter the number of gels:
 
       
Enter Desired Percent:
%
 
       
 
ml Total Volume
 
ml ddH2O
 
ml Acrylamide
 
ml 1.5M Tris pH 8.8
 
µl 10% SDS
 
µl 10% APS
 
µl TEMED
       
       
 
ml Stacking gel
 
ml ddH2O
 
ml % Acrylamide
 
ml 0.5M Tris pH 6.8
 
µl 10% SDS
 
µl 10% APS
 
µl TEMED
       
       

© 2007 Joseph T.E. Roland
www.Cytographica.com